nuclear factor kappa light chain enhancer of activated B cells
Pipoxolan suppresses the inflammatory factors of NF-κB, AP-1, and STATs, but activates the antioxidative factor Nrf2 in LPS-stimulated RAW 264.7 murine macrophage cells
Although pipoxolan (PIPO) is a smooth muscle relaxant, anti-inflammatory abilities have not been studied. Therefore, we investigated the molecular mechanism of anti-inflammatory PIPO in lipopolysaccharide (LPS) -induced RAW 264.7 macrophages. In this study, we used the MTT assay to evaluate cytotoxicity of the applied enzyme-linked immunosorbent assay to determine the inflammatory cytokines, and performed Western blotting to assess protein expression.
The results showed that PIPO significantly inhibited the production of cytokines, including nitric oxide, prostaglandin E2, tumor necrosis factor-α and interleukin-6. PIPO also suppresses pro-inflammatory mediator expression with inducible nitric oxide synthase and cyclooxygenase-2. Additionally, PIPO forbidden lane transcription factor of inflammatory several, including the inhibitor of kappa B / nuclear factor of κ chain enhancer light B cells (NF-kB), mitogen-activated protein kinase / activator protein-1 (AP-1),
Janus kinase / signal transducer and activator of transcription (STAT), and pulses like receptor 4 (TLR4) / serine / threonine kinase (Akt). In addition, effective PIPO activated nuclear factor erythroid 2-related factor 2 (Nrf2) / heme oxygenase-1 antioxidant pathway. Collectively, PIPO may attenuate the inflammatory effects through affecting LPS / TLR4 receptor binding; suppress the expression of anti-inflammatory transcription factor NF-kB, AP-1 and STAT; and activate the transcription factor Nrf2 antioxidant in LPS-stimulated RAW 264.7 mouse cells.
Pipoxolan suppresses the inflammatory factors of NF-κB, AP-1, and STATs, but activates the antioxidative factor Nrf2 in LPS-stimulated RAW 264.7 murine macrophage cells
Rhaponticin the effects of oxidative stress and inflammation in the diabetic retina through NRF2 / HO-1 / NF-kB signaling
Oxidative stress and inflammation has long been considered responsible for the development and progression of diabetic retinopathy. On the other hand, rhaponticin (RN) has received scientific attention for its wide range of pharmacological properties. Keeping all this in view, this study was conducted to investigate the potential protective effect of RN to the retina in diabetic rats. Rats were randomly divided into three groups: a control group of mice, rats diabetic groups, diabetes + RN (20 mg / kg body weight for 28 days via the oral route) mice groups. RN supplementation to significantly prevent diabetic mice lose weight reduction end, weekly fasting reduced blood glucose levels and HbA1c levels with a significant increase in serum levels of insulin.
Quantitative polymerase chain reaction and immunohistochemical analysis found increased regulation of Nrf2, NQO-1, HO-1 and upregulation of genes Keap1 and distribution of protein along with a significant level reduces malondialdehyde and increased activity of superoxide dismutase, catalase, and glutathione peroxidase in diabetes RN-treated mice compared to diabetic rats. In addition, treatment of diabetic mice with RN showed downregulated the expression of tumor necrosis factor-α, matrix metalloproteinase-2 and upregulated the expression of interleukin-10 (IL-10) and TIMP-1 in the retina. RN treatment decreased nuclear factor kappa-light-chain-enhancer of activated B cells and increased distribution of IL-10 protein distribution in the retina of diabetic rats. In addition, the RN treatment improved morphological changes were observed in the retina of diabetic rats.
Description: A Monoclonal antibody against Human NF-κB p65. The antibodies are raised in Mouse and are from clone 6H7. This antibody is applicable in WB, E
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IP, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IP, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against NF?B-p65 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A polyclonal antibody for detection of NF-?B p65 from Human, Mouse, Rat. This NF-?B p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide of NF-?B p65
Description: A polyclonal antibody for detection of NF-?B p65 from Human, Mouse, Rat. This NF-?B p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide of NF-?B p65
Description: A polyclonal antibody for detection of NF-?B p65 from Human, Mouse, Rat. This NF-?B p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide of NF-?B p65
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S281
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S281
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S281
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T505
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T505
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T505
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S536
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S536
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S536
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Monkey. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-acetylation site of K310 at AA range: 250-330
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Monkey. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-acetylation site of K310 at AA range: 250-330
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Monkey. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-acetylation site of K310 at AA range: 250-330
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, IP, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S276
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, IP, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S276
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, IP, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S276
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S311
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S311
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S311
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T435
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T435
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T435
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T254
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T254
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T254
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S468
×
Overall, these results provide clear evidence that treatment of diabetic rats with diabetic retinal changes RN attenuated through hypoglycemic, antioxidant and anti-inflammatory effects. Prolonged inflammatory response can lead to the development of from some chronic diseases, such as autoimmune disorders and the development of from natural therapeutic agent needed. A murine model was used to assess the effects of anti-inflammatory from the glucoside megastigmane, icariside B 2 (ICS B ), and the assessment was carried out in vitro , and in vivo.
