NF-κB

nuclear factor kappa light chain enhancer of activated B cells

Pipoxolan suppresses the inflammatory factors of NF-κB, AP-1, and STATs, but activates the antioxidative factor Nrf2 in LPS-stimulated RAW 264.7 murine macrophage cells

Pipoxolan suppresses the inflammatory factors of NF-κB, AP-1, and STATs, but activates the antioxidative factor Nrf2 in LPS-stimulated RAW 264.7 murine macrophage cells

Although pipoxolan (PIPO) is a smooth muscle relaxant, anti-inflammatory abilities have not been studied. Therefore, we investigated the molecular mechanism of anti-inflammatory PIPO in lipopolysaccharide (LPS) -induced RAW 264.7 macrophages. In this study, we used the MTT assay to evaluate cytotoxicity of the applied enzyme-linked immunosorbent assay to determine the inflammatory cytokines, and performed Western blotting to assess protein expression.

The results showed that PIPO significantly inhibited the production of cytokines, including nitric oxide, prostaglandin E2, tumor necrosis factor-α and interleukin-6. PIPO also suppresses pro-inflammatory mediator expression with inducible nitric oxide synthase and cyclooxygenase-2. Additionally, PIPO forbidden lane transcription factor of inflammatory several, including the inhibitor of kappa B / nuclear factor of κ chain enhancer light B cells (NF-kB), mitogen-activated protein kinase / activator protein-1 (AP-1),

Janus kinase / signal transducer and activator of transcription (STAT), and pulses like receptor 4 (TLR4) / serine / threonine kinase (Akt). In addition, effective PIPO activated nuclear factor erythroid 2-related factor 2 (Nrf2) / heme oxygenase-1 antioxidant pathway. Collectively, PIPO may attenuate the inflammatory effects through affecting LPS / TLR4 receptor binding; suppress the expression of anti-inflammatory transcription factor NF-kB, AP-1 and STAT; and activate the transcription factor Nrf2 antioxidant in LPS-stimulated RAW 264.7 mouse cells.

Pipoxolan suppresses the inflammatory factors of NF-κB, AP-1, and STATs, but activates the antioxidative factor Nrf2 in LPS-stimulated RAW 264.7 murine macrophage cells
Pipoxolan suppresses the inflammatory factors of NF-κB, AP-1, and STATs, but activates the antioxidative factor Nrf2 in LPS-stimulated RAW 264.7 murine macrophage cells

Rhaponticin the effects of oxidative stress and inflammation in the diabetic retina through NRF2 / HO-1 / NF-kB signaling

Oxidative stress and inflammation has long been considered responsible for the development and progression of diabetic retinopathy. On the other hand, rhaponticin (RN) has received scientific attention for its wide range of pharmacological properties. Keeping all this in view, this study was conducted to investigate the potential protective effect of RN to the retina in diabetic rats. Rats were randomly divided into three groups: a control group of mice, rats diabetic groups, diabetes + RN (20 mg / kg body weight for 28 days via the oral route) mice groups. RN supplementation to significantly prevent diabetic mice lose weight reduction end, weekly fasting reduced blood glucose levels and HbA1c levels with a significant increase in serum levels of insulin.

Quantitative polymerase chain reaction and immunohistochemical analysis found increased regulation of Nrf2, NQO-1, HO-1 and upregulation of genes Keap1 and distribution of protein along with a significant level reduces malondialdehyde and increased activity of superoxide dismutase, catalase, and glutathione peroxidase in diabetes RN-treated mice compared to diabetic rats. In addition, treatment of diabetic mice with RN showed downregulated the expression of tumor necrosis factor-α, matrix metalloproteinase-2 and upregulated the expression of interleukin-10 (IL-10) and TIMP-1 in the retina. RN treatment decreased nuclear factor kappa-light-chain-enhancer of activated B cells and increased distribution of IL-10 protein distribution in the retina of diabetic rats. In addition, the RN treatment improved morphological changes were observed in the retina of diabetic rats.

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

CN-01728R1 96T
EUR 572.4

Mouse nuclear factor-κB p65,NF-κB p65 ELISA Kit

CN-02606M1 96T
EUR 523.2

Mouse nuclear factor-κB p65,NF-κB p65 ELISA Kit

CN-02606M2 48T
EUR 344.4

Human nuclear factor-κB p65,NF-κB p65 ELISA Kit

CN-03540H1 96T
EUR 573.6

Human nuclear factor-κB p65,NF-κB p65 ELISA Kit

CN-03540H2 48T
EUR 394.8

Rat NF-κB p65(Nuclear Factor-κB P65) ELISA Kit

ER1187 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.875pg/ml

Mouse NF-κB p65(Nuclear Factor-κB p65) ELISA Kit

EM1231 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml

Rabbit nuclear factor-κB p65,NF-κB p65 ELISA Kit

GA-E0155RB-48T 48T
EUR 391.2

Rabbit nuclear factor-κB p65,NF-κB p65 ELISA Kit

GA-E0155RB-96T 96T
EUR 628.8

Rat nuclear factor-κB p65(NF-κB p65)ELISA Kit

GA-E0298RT-48T 48T
EUR 380.4

Rat nuclear factor-κB p65(NF-κB p65)ELISA Kit

GA-E0298RT-96T 96T
EUR 595.2

Mouse nuclear factor-κB p65(NF-κB p65)ELISA Kit

GA-E0534MS-48T 48T
EUR 403.2

Mouse nuclear factor-κB p65(NF-κB p65)ELISA Kit

GA-E0534MS-96T 96T
EUR 640.8

Human nuclear factor-κB p65(NF-κB p65)ELISA Kit

GA-E0683HM-48T 48T
EUR 346.8

Human nuclear factor-κB p65(NF-κB p65)ELISA Kit

GA-E0683HM-96T 96T
EUR 559.2

Human nuclear factor-κB p65(NF-κB p65)ELISA Kit

QY-E03142 96T
EUR 433.2

Rat nuclear factor-κB p65(NF-κB p65)ELISA Kit

QY-E11354 96T
EUR 433.2

Mouse nuclear factor-κB p65(NF-κB p65)ELISA Kit

QY-E21101 96T
EUR 433.2

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

YLA0513RA-48T 48T
EUR 465

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

YLA0513RA-96T 96T
EUR 600

Human Nuclear factor-κB p65(NF-κB p65)ELISA Kit

YLA0732HU-48T 48T
EUR 435

Human Nuclear factor-κB p65(NF-κB p65)ELISA Kit

YLA0732HU-96T 96T
EUR 562.5

Mouse nuclear factor-κB p65,NF-κB p65 ELISA Kit

YLA0507MO-48T 48T
EUR 465

Mouse nuclear factor-κB p65,NF-κB p65 ELISA Kit

YLA0507MO-96T 96T
EUR 600

NF-κB p65 (Ab-311) Antibody

33198-100ul 100ul
EUR 302.4

NF-κB p65 (Ab-311) Antibody

33198-50ul 50ul
EUR 224.4

Rat nuclear factor-κB p65,NF-κB p66 ELISA Kit

CN-01728R2 48T
EUR 393.6

NF-κB p65 (pS529) Cell ELISA Kit

abx595994-296tests 2 × 96 tests
EUR 848.4

NF-κB p65 (pS311) Cell ELISA Kit

abx596048-296tests 2 × 96 tests
EUR 848.4

NF-κB p65 (pS536) Cell ELISA Kit

abx596050-296tests 2 × 96 tests
EUR 848.4

Monoclonal NF-κB p65 Antibody, Clone: 6H7

APR08725G 0.1ml
EUR 580.8
Description: A Monoclonal antibody against Human NF-κB p65. The antibodies are raised in Mouse and are from clone 6H7. This antibody is applicable in WB, E

NF-κB p65 (Ab-311) Conjugated Antibody

C33198 100ul
EUR 476.4

Human Nuclear Factor Kappa B p65 (NF-κB p65) CLIA Kit

abx196082-96tests 96 tests
EUR 990

Human NF-κB p65(Nuclear Factor Kappa B p65) ELISA Kit

EH3423 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

NF-κB Reporter Kit (NF-κB Signaling Pathway)

60614 500 rxns.
EUR 650
Description: The NF-κB Reporter kit is designed for monitoring the activity of the NF-κB signaling pathway in the cultured cells. The kit contains transfection-ready NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.

Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway)

79268 500 rxns.
EUR 780
Description: The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

NF-κB p105/p50 Antibody

48117-100ul 100ul
EUR 399.6

NF-κB p105/p50 Antibody

48117-50ul 50ul
EUR 286.8

NF-κB p105/p50 Antibody

48171-100ul 100ul
EUR 399.6

NF-κB p105/p50 Antibody

48171-50ul 50ul
EUR 286.8

NF-κB p105/p50 Antibody

48268-100ul 100ul
EUR 399.6

NF-κB p105/p50 Antibody

48268-50ul 50ul
EUR 286.8

NF-κB Luciferase Reporter Lentivirus

79564 500 µl x 2
EUR 875
Description: The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by measuring the luciferase activity.

NF-κB eGFP Reporter Lentivirus

79926 500 µl x 2
EUR 820
Description: The NF-κB eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced GFP gene driven by the NF-κB response element located upstream of the minimal TATA promoter. After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by examining eGFP expression.

NF-kappaB p65/ Rat NF- kappaB p65 ELISA Kit

ELA-E0616r 96 Tests
EUR 1063.2

Human Nuclear factor-κB p105(NF-ΚB P105)ELISA Kit

YLA3992HU-48T 48T
EUR 435

Human Nuclear factor-κB p105(NF-ΚB P105)ELISA Kit

YLA3992HU-96T 96T
EUR 562.5

NF-κB p100 (Phospho-Ser872) Antibody

11919-100ul 100ul
EUR 302.4

NF-κB p100 (Phospho-Ser872) Antibody

11919-50ul 50ul
EUR 224.4

NF-κB p105 (Phospho-Ser903) Antibody

12407-100ul 100ul
EUR 302.4

NF-κB p105 (Phospho-Ser903) Antibody

12407-50ul 50ul
EUR 224.4

NF-κB p105 (Phospho-Ser923) Antibody

12408-100ul 100ul
EUR 302.4

NF-κB p105 (Phospho-Ser923) Antibody

12408-50ul 50ul
EUR 224.4

NF-κB p105/p50 Conjugated Antibody

C48171 100ul
EUR 476.4

Rat NF-κB p50 ELISA Kit

QY-E11915 96T
EUR 448.8

NF kappaB p65 antibody

70R-50315 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50316 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50317 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50318 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50319 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50320 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50321 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50322 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50323 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-50324 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-51627 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-51628 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-51629 100 ul
EUR 344.4
Description: Purified Polyclonal NF kappaB p65 antibody

NF kappaB p65 antibody

70R-51630 100 ul
EUR 292.8
Description: Purified Polyclonal NF kappaB p65 antibody

NF-kB p65 Antibody

48676-100ul 100ul
EUR 399.6

NF-kB p65 Antibody

48676-50ul 50ul
EUR 286.8

NF-kappaB p65 Antibody

20-abx141159
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

NF- kappaB p65 Antibody

ABF5006 100 ug
EUR 525.6

NF- kappaB p65 Antibody

ABF6387 100 ug
EUR 525.6

NF-kB p65 Antibody

AF6795 100ul
EUR 420

NF-kB p65 Antibody

BF0382 200ul
EUR 540

NF-kB p65 Antibody

DF7003 200ul
EUR 420

NF-kB p65 Antibody

AF5006 200ul
EUR 420

NF-kB p65 Antibody

AF6387 200ul
EUR 420

NF-kB p65 Antibody

AF0874 200ul
EUR 420

p65 Antibody / NF-kB

R20290-0.1ML 100ul
EUR 409

p65 Antibody NF-kB

R30293 100 ug
EUR 419

p65 Antibody NF-kB

R30754 100 ug
EUR 419

p65 Antibody / NF-kB

RQ4439 100ug
EUR 419

p65 Antibody / NF-kB

V9229-100UG 100ug
EUR 499
Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

p65 Antibody / NF-kB

V9229-20UG 20ug
EUR 219
Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

p65 Antibody / NF-kB

V9229SAF-100UG 100ug
EUR 499
Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

p65 Antibody / NF-kB

V9238-100UG 100ug
EUR 499
Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

p65 Antibody / NF-kB

V9238-20UG 20ug
EUR 219
Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

p65 Antibody / NF-kB

V9238SAF-100UG 100ug
EUR 499
Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

NF-κB Reporter Cellular Assay Pack (CHOK1)

79325 2 vials
EUR 1245
Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- CHO-K1 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine. _x000D_Additionally, the pack includes cell culture medium (Thaw Medium 3) that has been optimized for use with CHO-K1 cells. Thaw Medium 3 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

NF-κB Reporter Cellular Assay Pack (HCT116)

79326 2 vials
EUR 1245
Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D__x000D_

NF-κB Reporter Cellular Assay Pack (HEK293)

79327 2 vials
EUR 1515
Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)-HEK293 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 1) that has been optimized for use with HEK293 cells. Thaw Medium 1 includes 10% fetal bovine serum, non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_

NF-κB Reporter (Luc) - HCT116 Cell Line

60623 2 vials
EUR 1095
Description: NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling
in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-κB activities as a
result of targeted genetic mutation(s).

NF-κB reporter (Luc) - HEK293 Cell line

60650 2 vials
EUR 1365
Description: The NF-κB reporter (Luc) HEK293 cell line is designed to monitor nuclear factor Kappa B (NF-κB) activity. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been functionally validated in response to human TNF-α, IL-1β, and IL-17.

NF-kB p65 Rabbit pAb

A0450-100ul 100 ul
EUR 369.6

NF-kB p65 Rabbit pAb

A0450-200ul 200 ul
EUR 550.8

NF-kB p65 Rabbit pAb

A0450-20ul 20 ul Ask for price

NF-kB p65 Rabbit pAb

A0450-50ul 50 ul Ask for price

NF-kB p65 Mouse mAb

A10609 20 μL
EUR 18

NF-kB p65 Mouse mAb

A10609-100ul 100 ul
EUR 460.8

NF-kB p65 Mouse mAb

A10609-200ul 200 ul
EUR 664.8

NF-kB p65 Mouse mAb

A10609-20ul 20 ul Ask for price

NF-kB p65 Mouse mAb

A10609-50ul 50 ul
EUR 318

NF-kB p65 Rabbit pAb

A11202-100ul 100 ul
EUR 369.6

NF-kB p65 Rabbit pAb

A11202-200ul 200 ul
EUR 550.8

NF-kB p65 Rabbit pAb

A11202-20ul 20 ul
EUR 219.6

NF-kB p65 Rabbit pAb

A11202-50ul 50 ul
EUR 267.6

NF-kB p65 Rabbit pAb

A11204-100ul 100 ul
EUR 369.6

NF-kB p65 Rabbit pAb

A11204-200ul 200 ul
EUR 550.8

NF-kB p65 Rabbit pAb

A11204-20ul 20 ul
EUR 219.6

NF-kB p65 Rabbit pAb

A11204-50ul 50 ul
EUR 267.6

NF kappa B p65 antibody

70R-13789 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal Human NFKB p65 antibody

NF-kB p65 Rabbit pAb

A2547-100ul 100 ul
EUR 369.6

NF-kB p65 Rabbit pAb

A2547-200ul 200 ul
EUR 550.8

NF-kB p65 Rabbit pAb

A2547-20ul 20 ul
EUR 219.6

NF-kB p65 Rabbit pAb

A2547-50ul 50 ul
EUR 267.6

NF?B-p65 Polyclonal Antibody

ABP56027-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S281

NF?B-p65 Polyclonal Antibody

ABP56027-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S281

NF?B-p65 Polyclonal Antibody

ABP56027-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S281

NF-kappaB p65 (AcK122) Antibody

20-abx141157
  • EUR 460.80
  • EUR 727.20
  • EUR 276.00
  • 100 ul
  • 200 ul
  • 30 ul

NF-kappaB p65 (AcK218) Antibody

20-abx141158
  • EUR 460.80
  • EUR 727.20
  • EUR 276.00
  • 100 ul
  • 200 ul
  • 30 ul

NF?B-p65 Polyclonal Antibody

ABP51949-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T505

NF?B-p65 Polyclonal Antibody

ABP51949-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T505

NF?B-p65 Polyclonal Antibody

ABP51949-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of T505

NF?B-p65 Polyclonal Antibody

ABP51950-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S536

NF?B-p65 Polyclonal Antibody

ABP51950-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S536

NF?B-p65 Polyclonal Antibody

ABP51950-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S536

NF?B-p65 Polyclonal Antibody

ABP51951-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Monkey. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-acetylation site of K310 at AA range: 250-330

NF?B-p65 Polyclonal Antibody

ABP51951-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Monkey. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-acetylation site of K310 at AA range: 250-330

NF?B-p65 Polyclonal Antibody

ABP51951-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Monkey. This NF?B-p65 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-acetylation site of K310 at AA range: 250-330

NF?B-p65 Polyclonal Antibody

ABP51952-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529

NF?B-p65 Polyclonal Antibody

ABP51952-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529

NF?B-p65 Polyclonal Antibody

ABP51952-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse, Rat, Monkey. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529

NF?B-p65 Polyclonal Antibody

ABP51953-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529

NF?B-p65 Polyclonal Antibody

ABP51953-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of NF?B-p65 from Human, Mouse. This NF?B-p65 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the non-phosphorylation site of S529

Overall, these results provide clear evidence that treatment of diabetic rats with diabetic retinal changes RN attenuated through hypoglycemic, antioxidant and anti-inflammatory effects. Prolonged inflammatory response can lead to the development of from some chronic diseases, such as autoimmune disorders and the development of from natural therapeutic agent needed. A murine model was used to assess the effects of anti-inflammatory from the glucoside megastigmane, icariside B 2 (ICS B ), and the assessment was carried out in vitro , and in vivo.

May 2023
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