Glyoxal and Methylglyoxal as E-cigarette Vapor Ingredients-Induced Pro-Inflammatory Cytokine and Mucins Expression in Human Nasal Epithelial Cells

Glyoxal and Methylglyoxal as E-cigarette Vapor Ingredients-Induced Pro-Inflammatory Cytokine and Mucins Expression in Human Nasal Epithelial Cells

Background: Glyoxal (GO) and methylglyoxal (MGO) is among the majority of the toxic compounds emitted by electronic cigarettes (e-cigarettes) and cigarette smoke tobacco regularly. respiratory diseases presented mucus over production as their main pathophysiologic features. However, the effects of GO and MGO in pro-inflammatory cytokines and mucin expression in human nasal epithelial cells, as well as the underlying signaling pathway, has not been studied.


Objective: This study was to determine whether the GO and MGO induce pro-inflammatory cytokines, and expression of MUC5AC / 5B via MAPK (MAPK) and nuclear factor-kappa-light-chain-enhancer activated B cells (NF kB) signaling pathway.


Methods: The effect of GO and MGO in pro-inflammatory cytokines, mucins expression and signaling pathways of GO and MGO were investigated using enzyme immunoassay water-soluble tetrazolium salt-1, and immunoblot analysis with specific inhibitors and small interfering RNA.


Results: GO and MGO did not affect cell viability up to 2 mM in human nasal epithelial cells. GO and MGO increased production of pro-inflammatory such as interleukin (IL) -1β and IL-6) and MUC5AC / 5B. In addition, GO and MGO was significantly activated extracellular signal-regulated kinase 1/2 (ERK1 / 2), p38 MAPK and NF-kB. Do ERK1 / 2, p38 MAPK and NF-kB signaling pathway involved in the GO and MGO-induced production of pro-inflammatory cytokines (IL-1β and IL-6) and MUC5AC / 5B, we used specific inhibitors and siRNA transfection. This is significantly suppressed Go and MGO-induced expression of pro-inflammatory cytokines (IL-1β and IL-6) and MUC5AC / 5B.

Conclusions: GO and MGO-induced pro-inflammatory cytokines and expression of MUC5AC / 5B through ERK1 / 2, p38 MAPK and NF-kB in human nasal epithelial cells. These results indicate that GO and MGO may be involved in airway diseases related to hypersecretion of mucus.

Glyoxal and Methylglyoxal as E-cigarette Vapor Ingredients-Induced Pro-Inflammatory Cytokine and Mucins Expression in Human Nasal Epithelial Cells
Glyoxal and Methylglyoxal as E-cigarette Vapor Ingredients-Induced Pro-Inflammatory Cytokine and Mucins Expression in Human Nasal Epithelial Cells

Acyclic Triterpenoid Isolated from Alpinia katsumadai Relieves Chronic Formalin-Induced Rat Paw Inflammation by Inhibiting the phosphorylation of ERK and NF-kB

Chronic and excessive inflammation can damage organs and cause a host of inflammatory diseases such as inflammatory bowel disease, asthma, and rheumatoid arthritis. In this study, we investigated the anti-inflammatory effects of Alpinia katsumadai derived seed-pentahydroxy 2,3,5,22,23-2,6,10,15,19,23-hexamethyl-tetracosa-6,10,14, 18-tetraene (IPM) using lipopolysaccharide (LPS) -stimulated J774 cells and chronic foot rat model of formalin-induced inflammation. In vitro results showed that PHT showed no cytotoxicity and decreased LPS-induced NO secretion.

In addition, PHT inhibit LPS-induced inducible NO synthase (iNOS) and cyclooxygenase 2 expression (COX2) protein. Quantitative real-time PCR results showed that PHT downregulated gene expression of proinflammatory cytokine interleukin-1β (IL-1β) and interleukin-6 (IL-6) but not tumor necrosis factor α (TNF-α). IPM inhibit LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK) and nuclear factor of kappa light chain enhancer activated B cells (NF-kB). In the mouse model, oral administration of 50 mg / kg PHT significantly reduced both the thickness and volume of mouse feet.

These results indicate that IPM has potential anti-inflammatory effects and should be considered as a possible functional material.Human podocytes (HPC) plays an important role in the pathogenesis of kidney disease. In this context, angiotensin II (Ang II) and nuclear factor kappa-light-chain-enhancer activated B cells (NF) plays an important role in Podosit injury.

MLK3 (Phospho- Thr277+ Ser281) Antibody

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Phospho-MLK3 (Thr277+Ser281) Antibody

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EUR 540

Phospho-MLK3 (Thr277+Ser281) Antibody

AF8079-100ul 100ul
EUR 350

Phospho-MLK3 (Thr277+Ser281) Antibody

AF8079-200ul 200ul
EUR 450

NFκB-p65 (phospho Ser281) Polyclonal Antibody

E20-61029 100ug
EUR 225
Description: Available in various conjugation types.

NFκB-p65 (phospho Ser281) Polyclonal Antibody

E44H00253 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

MLK3 (Phospho Thr277+Ser281) rabbit pAb

E28PP1580 100μl
EUR 255
Description: Available in various conjugation types.

Phospho-NF-kB p65 (Ser281) Antibody

AF3393 200ul
EUR 420

Phospho-NF-kB p65 (Ser281) Antibody

AF3393-100ul 100ul
EUR 280

Phospho-NF-kB p65 (Ser281) Antibody

AF3393-200ul 200ul
EUR 350

Phospho- NF- kappaB p65 (Ser281) Antibody

ABF3393 100 ug
EUR 525.6

MLK3 (Phospho Thr277+Ser281) rabbit pAb

E44H14661 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Phospho-NF- kappaB p65 (Ser281) Antibody

E18-3393-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Phospho-NF- kappaB p65 (Ser281) Antibody

E18-3393-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

NF?B-p65 (phospho Ser281) Polyclonal Antibody

ABP56026-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of NF?B-p65 phospho Ser281) from Human, Mouse, Rat. This NF?B-p65 phospho Ser281) antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the phosphorylation site of S281

NF?B-p65 (phospho Ser281) Polyclonal Antibody

ABP56026-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of NF?B-p65 phospho Ser281) from Human, Mouse, Rat. This NF?B-p65 phospho Ser281) antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the phosphorylation site of S281

NF?B-p65 (phospho Ser281) Polyclonal Antibody

ABP56026-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of NF?B-p65 phospho Ser281) from Human, Mouse, Rat. This NF?B-p65 phospho Ser281) antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human NF?B-p65 around the phosphorylation site of S281

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AF8079-BP 1mg
EUR 234

Phospho-NF-KB p65 (Ser281) Rabbit pAb

E2310314 100ul
EUR 225
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

NFκB-p65 (phospho Ser281) Rabbit Polyclonal Antibody

ES7025-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against NF?B-p65 (phospho Ser281) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA

NFκB-p65 (phospho Ser281) Rabbit Polyclonal Antibody

ES7025-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against NF?B-p65 (phospho Ser281) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA

Phospho-NF kappaB p65 (Ser281) Blocking Peptide

AF3393-BP 1mg
EUR 234

Rabbit polyclonal NF-kB p65 (Ser281) antibody(Phospho-specific)

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Rabbit Polyclonal NF- kappaB p65 (Ser281) Antibody (Phospho-specific)

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NFkB p65 (Phospho-Ser281) Antibody

20-abx012581
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NFkB p65 (Phospho-Ser281) Antibody

abx012581-100g 100 µg
EUR 237.5

NFkB p65 (Phospho-Ser281) Antibody

abx012581-10g 10 µg
EUR 43.75

NFkB p65 (Phospho-Ser281) Antibody

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EUR 312.5

ATM (phospho Ser1981) Antibody

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ATM (Phospho- Ser1981) Antibody

ABF4120 100 ug
EUR 525.6

ATM (Phospho- Ser1981) Antibody

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ATM (Phospho- Ser1981) Antibody

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ATM (Phospho- Ser1987) Antibody

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eNOS (Phospho- Ser1179) Antibody

ABF8086 100 ug
EUR 525.6

TIE2 (Phospho- Ser1119) Antibody

ABF8193 100 ug
EUR 525.6

CD91 (Phospho- Ser4520) Antibody

ABF8207 100 ug
EUR 525.6

nNOS (Phospho- Ser1417) Antibody

ABF8231 100 ug
EUR 525.6

MUC1 (Phospho- Ser1227) Antibody

ABF8289 100 ug
EUR 525.6

LRP6 (Phospho- Ser1490) Antibody

ABF8344 100 ug
EUR 525.6

TSC2 (Phospho- Ser1254) Antibody

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EUR 525.6

IRS2 (Phospho- Ser1100) Antibody

ABF8383 100 ug
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eNOS Antibody (phospho Ser1177)

GWB-A903B6 0.1 mg Ask for price

eNOS Antibody (phospho Ser1177)

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Rb (Phospho- Ser795) Antibody

E11-7209A 100μg/100μl
EUR 225
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Rb Antibody (phospho Ser249)

GWB-438CD0 0.05 ml Ask for price

Rb Antibody (phospho Ser780)

GWB-48818B 0.05 ml Ask for price

Phospho- GR (Ser203) Antibody

ABF0042 100 ug
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Phospho- GR (Ser211) Antibody

ABF2004 100 ug
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Phospho- GR (Ser226) Antibody

ABF3066 100 ug
EUR 525.6

Phospho- GR (Ser203) Antibody

ABF3067 100 ug
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Phospho- Rb (Ser608) Antibody

ABF3726 100 ug
EUR 525.6

Rb (phospho Ser608) Antibody

GWB-C86D15 0.05 mg Ask for price

IGF2R (Phospho- Ser2484) Antibody

ABF8068 100 ug
EUR 525.6

TOP2A (Phospho- Ser1213) Antibody

ABF8090 100 ug
EUR 525.6

BRCA1 (Phospho- Ser1387) Antibody

ABF8202 100 ug
EUR 525.6

BRCA1 (Phospho- Ser1497) Antibody

ABF8204 100 ug
EUR 525.6

CUTL1 (Phospho- Ser1215) Antibody

ABF8262 100 ug
EUR 525.6

CUTL1 (Phospho- Ser1237) Antibody

ABF8263 100 ug
EUR 525.6

53BP1 (Phospho- Ser1778) Antibody

ABF8337 100 ug
EUR 525.6

PLCG1 (Phospho- Ser1248) Antibody

ABF8390 100 ug
EUR 525.6

TOP2A (Phospho- Ser1337) Antibody

ABF8516 100 ug
EUR 525.6

p53 (Phospho- Ser315) Antibody

E11-7181A 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Tau Antibody (phospho Ser396)

GWB-28711E 0.05 ml Ask for price

FAK Antibody (phospho Ser732)

GWB-2C7E7E 0.05 ml Ask for price

Tau Antibody (phospho Ser409)

GWB-10EF47 0.05 ml Ask for price

NF2 Antibody (phospho Ser518)

GWB-3996E4 0.1 mg Ask for price

Tau Antibody (phospho Ser199)

GWB-1AFEA9 0.05 ml Ask for price

HSL (Phospho- Ser660) Antibody

ABF8026 100 ug
EUR 525.6

ATM (Phospho- Ser367) Antibody

ABF8099 100 ug
EUR 525.6

ATM (Phospho- Ser183) Antibody

ABF8100 100 ug
EUR 525.6

Met (Phospho- Ser985) Antibody

ABF8121 100 ug
EUR 525.6

KIT (Phospho- Ser746) Antibody

ABF8162 100 ug
EUR 525.6

ZAK (Phospho- Ser165) Antibody

ABF8195 100 ug
EUR 525.6

FAK (Phospho- Ser732) Antibody

ABF8227 100 ug
EUR 525.6

Fos (Phospho- Ser374) Antibody

ABF8270 100 ug
EUR 525.6

p53 (Phospho- Ser392) Antibody

ABF8295 100 ug
EUR 525.6

Tel (Phospho- Ser257) Antibody

ABF8325 100 ug
EUR 525.6

XPA (Phospho- Ser196) Antibody

ABF8335 100 ug
EUR 525.6

BTK (Phospho- Ser179) Antibody

ABF8361 100 ug
EUR 525.6

FAK (Phospho- Ser722) Antibody

ABF8373 100 ug
EUR 525.6

ATM (Phospho- Ser794) Antibody

ABF8411 100 ug
EUR 525.6

Cot (Phospho- Ser400) Antibody

ABF8469 100 ug
EUR 525.6

p73 (Phospho- Ser388) Antibody

ABF8500 100 ug
EUR 525.6

VR1 (Phospho- Ser503) Antibody

ABF8520 100 ug
EUR 525.6

Lck Antibody (phospho Ser158)

GWB-AC0250 0.05 ml Ask for price

FAK Antibody (phospho Ser722)

GWB-9A369B 0.05 ml Ask for price

Tau Antibody (phospho Ser262)

GWB-633D05 0.05 ml Ask for price

p53 Antibody (phospho Ser392)

GWB-7BADB0 0.1 ml Ask for price

H2A.X Antibody (phospho Ser139)

GWB-E2A6B6 0.05 mg Ask for price

Tau Antibody (phospho Ser214)

GWB-EFF526 0.05 ml Ask for price

p53 Antibody (phospho Ser392)

GWB-78870B 0.1 mg Ask for price

FAK Antibody (phospho Ser910)

GWB-F41A25 0.05 ml Ask for price

Tau Antibody (phospho Ser356)

GWB-CF7666 0.05 ml Ask for price

BTK (phospho Ser180) Antibody

GWB-C6A7DB 0.05 ml Ask for price

NF2 Antibody (phospho Ser518)

GWB-D461F1 0.05 mg Ask for price

Acinus (Phospho- Ser1180) Antibody

ABF8237 100 ug
EUR 525.6

POLR2A (Phospho- Ser1616) Antibody

ABF8348 100 ug
EUR 525.6

TOPBP1 (Phospho- Ser1159) Antibody

ABF8517 100 ug
EUR 525.6

Rb Antibody (phospho Ser807/Ser811)

GWB-7E135C 0.05 ml Ask for price

RIP3 (Phospho- Ser304 ) Antibody

13283-100ul 100ul
EUR 302.4

RIP3 (Phospho- Ser304 ) Antibody

13283-50ul 50ul
EUR 224.4

RIP3 (Phospho- Ser316 ) Antibody

12840-100ul 100ul
EUR 302.4

RIP3 (Phospho- Ser316 ) Antibody

12840-50ul 50ul
EUR 224.4

RIP3 (Phospho- Ser339) Antibody

12841-100ul 100ul
EUR 302.4

RIP3 (Phospho- Ser339) Antibody

12841-50ul 50ul
EUR 224.4

SMC1 Antibody (phospho Ser966)

GWB-29478F 0.05 mg Ask for price

MEK1 Antibody (phospho Ser298)

GWB-5B25D8 0.05 ml Ask for price

PTEN Antibody (phospho Ser385)

GWB-456AC1 0.05 ml Ask for price

Gab2 (Phospho- Ser623) Antibody

ABF5427 100 ug
EUR 525.6

Daxx (Phospho- Ser668) Antibody

ABF5441 100 ug
EUR 525.6

BACE (Phospho- Ser498) Antibody

ABF8003 100 ug
EUR 525.6

CTIP (Phospho- Ser327) Antibody

ABF8004 100 ug
EUR 525.6

RAD9 (Phospho- Ser272) Antibody

ABF8019 100 ug
EUR 525.6

RhoA (Phospho- Ser188) Antibody

ABF8020 100 ug
EUR 525.6

TLK1 (Phospho- Ser764) Antibody

ABF8029 100 ug
EUR 525.6

RBL2 (Phospho- Ser952) Antibody

ABF8036 100 ug
EUR 525.6

CFTR (Phospho- Ser737) Antibody

ABF8042 100 ug
EUR 525.6

HSF1 (Phospho- Ser307) Antibody

ABF8066 100 ug
EUR 525.6

MAP4 (Phospho- Ser696) Antibody

ABF8075 100 ug
EUR 525.6

PKD2 (Phospho- Ser812) Antibody

ABF8092 100 ug
EUR 525.6

CREB (Phospho- Ser111) Antibody

ABF8109 100 ug
EUR 525.6

eNOS (Phospho- Ser114) Antibody

ABF8111 100 ug
EUR 525.6

eNOS (Phospho- Ser633) Antibody

ABF8112 100 ug
EUR 525.6

LKB1 (Phospho- Ser334) Antibody

ABF8164 100 ug
EUR 525.6

MSK2 (Phospho- Ser196) Antibody

ABF8172 100 ug
EUR 525.6

PKCD (Phospho- Ser664) Antibody

ABF8182 100 ug
EUR 525.6

PKG2 (Phospho- Ser126) Antibody

ABF8184 100 ug
EUR 525.6

RSK3 (Phospho- Ser360) Antibody

ABF8188 100 ug
EUR 525.6

TBK1 (Phospho- Ser172) Antibody

ABF8190 100 ug
EUR 525.6

APLF (Phospho- Ser116) Antibody

ABF8241 100 ug
EUR 525.6

BORA (Phospho- Ser497) Antibody

ABF8249 100 ug
EUR 525.6

CDX2 (Phospho- Ser283) Antibody

ABF8253 100 ug
EUR 525.6

FKHR (Phospho- Ser249) Antibody

ABF8271 100 ug
EUR 525.6

GAIP (Phospho- Ser151) Antibody

ABF8274 100 ug
EUR 525.6

MAP2 (Phospho- Ser136) Antibody

ABF8281 100 ug
EUR 525.6

MLK3 (Phospho- Ser674) Antibody

ABF8288 100 ug
EUR 525.6

PAK6 (Phospho- Ser165) Antibody

ABF8297 100 ug
EUR 525.6

TAB1 (Phospho- Ser438) Antibody

ABF8324 100 ug
EUR 525.6

AKT1 (Phospho- Ser473) Antibody

ABF8355 100 ug
EUR 525.6

AKT1 (Phospho- Ser473) Antibody

ABF8356 100 ug
EUR 525.6

AQP2 (Phospho- Ser261) Antibody

ABF8360 100 ug
EUR 525.6

CDH1 (Phospho- Ser844) Antibody

ABF8365 100 ug
EUR 525.6

CDK5 (Phospho- Ser159) Antibody

ABF8366 100 ug
EUR 525.6

E2F1 (Phospho- Ser364) Antibody

ABF8369 100 ug
EUR 525.6

ETS1 (Phospho- Ser251) Antibody

ABF8371 100 ug
EUR 525.6

ETS1 (Phospho- Ser282) Antibody

ABF8372 100 ug
EUR 525.6

IRF5 (Phospho- Ser437) Antibody

ABF8382 100 ug
EUR 525.6

RBL2 (Phospho- Ser639) Antibody

ABF8401 100 ug
EUR 525.6

CD66 (Phospho- Ser508) Antibody

ABF8414 100 ug
EUR 525.6

Chk2 (Phospho- Ser379) Antibody

ABF8415 100 ug
EUR 525.6

ROR2 (Phospho- Ser449) Antibody

ABF8451 100 ug
EUR 525.6

CD88 (Phospho- Ser327) Antibody

ABF8468 100 ug
EUR 525.6

More recently, transmembrane proteins (Tmem) 63C, a member of Tmem-family found to be expressed in the kidney and associated with Podosit function. In this study, we analyzed the expression and regulation of functional Tmem63c impact on cell viability and apoptosis in HPC in the context of activation of Ang II.

Tony

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